We are pleased to announce that a new and improved version of PrimerQuest® has been launched. PrimerQuest is our foundational PCR and qPCR design tool and, hence, one of our most widely used SciTools applications. As well as serving the primer design needs of researchers who perform sequencing and endpoint PCR, it serves customers whose research involves qPCR in species other than human, mouse, and rat (for human, mouse, and rat use the IDT PrimeTime® Assay Library Selection Tool).
Science website: how to set up a PCR lab, devil is in the details article
Design successful SYBR® Green, TaqMan®, HRMA Primers, MethyLight TaqMan®, LNA™ spiked TaqMan®, molecular beacons for both standard and NASBA® assays, Scorpions® and FRET assays by automatically interpreting BLAST results and avoiding template structures.
Dr Aurita Menezes, qPCR Product Manager at IDT, explains the value of knowing your primer and probe sequences, how PrimeTime® qPCR Assays are designed to avoid SNPs, and the effect of including the ZEN™ Double-Quenched Probe in your assay.